Detection of influenza A(H1N1)v virus by real-time RT-PCR.
Identifieur interne : 000410 ( Main/Exploration ); précédent : 000409; suivant : 000411Detection of influenza A(H1N1)v virus by real-time RT-PCR.
Auteurs : M. Panning [Allemagne] ; M. Eickmann ; O. Landt ; M. Monazahian ; S. Olschl Ger ; S. Baumgarte ; U. Reischl ; J J Wenzel ; H H Niller ; S. Günther ; B. Hollmann ; D. Huzly ; J F Drexler ; A. Helmer ; S. Becker ; B. Matz ; Am Eis-Hübinger ; C. DrostenSource :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2009.
Descripteurs français
- KwdFr :
- Allemagne (épidémiologie), Flambées de maladies (), Grippe humaine (diagnostic), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains, RT-PCR (), Reproductibilité des résultats, Sensibilité et spécificité, Sous-type H1N2 du virus de la grippe A (génétique), Sous-type H1N2 du virus de la grippe A (isolement et purification).
- MESH :
- diagnostic : Grippe humaine.
- génétique : Sous-type H1N2 du virus de la grippe A.
- isolement et purification : Sous-type H1N2 du virus de la grippe A.
- virologie : Grippe humaine.
- épidémiologie : Allemagne, Grippe humaine.
- Flambées de maladies, Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Disease Outbreaks (statistics & numerical data), Germany (epidemiology), Humans, Influenza A Virus, H1N2 Subtype (genetics), Influenza A Virus, H1N2 Subtype (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (epidemiology), Influenza, Human (virology), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity.
- MESH :
- geographic , epidemiology : Germany.
- diagnosis : Influenza, Human.
- epidemiology : Influenza, Human.
- genetics : Influenza A Virus, H1N2 Subtype.
- isolation & purification : Influenza A Virus, H1N2 Subtype.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- statistics & numerical data : Disease Outbreaks.
- virology : Influenza, Human.
- Humans, Reproducibility of Results, Sensitivity and Specificity.
Abstract
Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference samples including seasonal human influenza A virus H1N1 and H3N2, highly pathogenic avian influenza A virus H5N1 and porcine influenza A virus H1N1, H1N2 and H3N2 samples. The real-time RT-PCR assay for the influenza A matrix gene recommended in 2007 by the World Health Organization was modified to work under the same reaction conditions as the influenza A(H1N1)v virus-specific test. Both assays were equally sensitive. Clinical applicability of both assays was demonstrated by screening of almost 2,000 suspected influenza (H1N1)v specimens, which included samples from the first cases of pandemic H1N1 influenza imported to Germany. Measuring influenza A(H1N1)v virus concentrations in 144 laboratory-confirmed samples yielded a median of 4.6 log RNA copies/ml. The new methodology proved its principle and might assist public health laboratories in the upcoming influenza pandemic.
PubMed: 19758541
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Panning</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology, University of Freiburg, Freiburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Virology, University of Freiburg, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Eickmann, M" sort="Eickmann, M" uniqKey="Eickmann M" first="M" last="Eickmann">M. Eickmann</name></author><author><name sortKey="Landt, O" sort="Landt, O" uniqKey="Landt O" first="O" last="Landt">O. Landt</name></author><author><name sortKey="Monazahian, M" sort="Monazahian, M" uniqKey="Monazahian M" first="M" last="Monazahian">M. Monazahian</name></author><author><name sortKey="Olschl Ger, S" sort="Olschl Ger, S" uniqKey="Olschl Ger S" first="S" last="Olschl Ger">S. Olschl Ger</name></author><author><name sortKey="Baumgarte, S" sort="Baumgarte, S" uniqKey="Baumgarte S" first="S" last="Baumgarte">S. Baumgarte</name></author><author><name sortKey="Reischl, U" sort="Reischl, U" uniqKey="Reischl U" first="U" last="Reischl">U. Reischl</name></author><author><name sortKey="Wenzel, J J" sort="Wenzel, J J" uniqKey="Wenzel J" first="J J" last="Wenzel">J J Wenzel</name></author><author><name sortKey="Niller, H H" sort="Niller, H H" uniqKey="Niller H" first="H H" last="Niller">H H Niller</name></author><author><name sortKey="Gunther, S" sort="Gunther, S" uniqKey="Gunther S" first="S" last="Günther">S. Günther</name></author><author><name sortKey="Hollmann, B" sort="Hollmann, B" uniqKey="Hollmann B" first="B" last="Hollmann">B. Hollmann</name></author><author><name sortKey="Huzly, D" sort="Huzly, D" uniqKey="Huzly D" first="D" last="Huzly">D. Huzly</name></author><author><name sortKey="Drexler, J F" sort="Drexler, J F" uniqKey="Drexler J" first="J F" last="Drexler">J F Drexler</name></author><author><name sortKey="Helmer, A" sort="Helmer, A" uniqKey="Helmer A" first="A" last="Helmer">A. Helmer</name></author><author><name sortKey="Becker, S" sort="Becker, S" uniqKey="Becker S" first="S" last="Becker">S. Becker</name></author><author><name sortKey="Matz, B" sort="Matz, B" uniqKey="Matz B" first="B" last="Matz">B. Matz</name></author><author><name sortKey="Eis Hubinger, Am" sort="Eis Hubinger, Am" uniqKey="Eis Hubinger A" first="Am" last="Eis-Hübinger">Am Eis-Hübinger</name></author><author><name sortKey="Drosten, C" sort="Drosten, C" uniqKey="Drosten C" first="C" last="Drosten">C. Drosten</name></author></analytic><series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title><idno type="eISSN">1560-7917</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Outbreaks (statistics & numerical data)</term><term>Germany (epidemiology)</term><term>Humans</term><term>Influenza A Virus, H1N2 Subtype (genetics)</term><term>Influenza A Virus, H1N2 Subtype (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (epidemiology)</term><term>Influenza, Human (virology)</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allemagne (épidémiologie)</term><term>Flambées de maladies ()</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (virologie)</term><term>Grippe humaine (épidémiologie)</term><term>Humains</term><term>RT-PCR ()</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Sous-type H1N2 du virus de la grippe A (génétique)</term><term>Sous-type H1N2 du virus de la grippe A (isolement et purification)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Germany</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H1N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Disease Outbreaks</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Allemagne</term><term>Grippe humaine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Flambées de maladies</term><term>Humains</term><term>RT-PCR</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Allemagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference samples including seasonal human influenza A virus H1N1 and H3N2, highly pathogenic avian influenza A virus H5N1 and porcine influenza A virus H1N1, H1N2 and H3N2 samples. The real-time RT-PCR assay for the influenza A matrix gene recommended in 2007 by the World Health Organization was modified to work under the same reaction conditions as the influenza A(H1N1)v virus-specific test. Both assays were equally sensitive. Clinical applicability of both assays was demonstrated by screening of almost 2,000 suspected influenza (H1N1)v specimens, which included samples from the first cases of pandemic H1N1 influenza imported to Germany. Measuring influenza A(H1N1)v virus concentrations in 144 laboratory-confirmed samples yielded a median of 4.6 log RNA copies/ml. The new methodology proved its principle and might assist public health laboratories in the upcoming influenza pandemic.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19758541</PMID><DateCompleted><Year>2009</Year><Month>12</Month><Day>02</Day></DateCompleted><DateRevised><Year>2013</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1560-7917</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>36</Issue><PubDate><Year>2009</Year><Month>Sep</Month><Day>10</Day></PubDate></JournalIssue><Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title><ISOAbbreviation>Euro Surveill.</ISOAbbreviation></Journal><ArticleTitle>Detection of influenza A(H1N1)v virus by real-time RT-PCR.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">19329</ELocationID><Abstract><AbstractText>Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference samples including seasonal human influenza A virus H1N1 and H3N2, highly pathogenic avian influenza A virus H5N1 and porcine influenza A virus H1N1, H1N2 and H3N2 samples. The real-time RT-PCR assay for the influenza A matrix gene recommended in 2007 by the World Health Organization was modified to work under the same reaction conditions as the influenza A(H1N1)v virus-specific test. Both assays were equally sensitive. Clinical applicability of both assays was demonstrated by screening of almost 2,000 suspected influenza (H1N1)v specimens, which included samples from the first cases of pandemic H1N1 influenza imported to Germany. Measuring influenza A(H1N1)v virus concentrations in 144 laboratory-confirmed samples yielded a median of 4.6 log RNA copies/ml. The new methodology proved its principle and might assist public health laboratories in the upcoming influenza pandemic.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Panning</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Virology, University of Freiburg, Freiburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eickmann</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Landt</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Monazahian</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Olschläger</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Baumgarte</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Reischl</LastName><ForeName>U</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Wenzel</LastName><ForeName>J J</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Niller</LastName><ForeName>H H</ForeName><Initials>HH</Initials></Author><Author ValidYN="Y"><LastName>Günther</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hollmann</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Huzly</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Drexler</LastName><ForeName>J F</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Helmer</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Becker</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Matz</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Eis-Hübinger</LastName><ForeName>Am</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Drosten</LastName><ForeName>C</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>09</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Sweden</Country><MedlineTA>Euro Surveill</MedlineTA><NlmUniqueID>100887452</NlmUniqueID><ISSNLinking>1025-496X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName><QualifierName UI="Q000706" MajorTopicYN="Y">statistics & numerical data</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057221" MajorTopicYN="N">Influenza A Virus, H1N2 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19758541</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><noCountry><name sortKey="Baumgarte, S" sort="Baumgarte, S" uniqKey="Baumgarte S" first="S" last="Baumgarte">S. Baumgarte</name><name sortKey="Becker, S" sort="Becker, S" uniqKey="Becker S" first="S" last="Becker">S. Becker</name><name sortKey="Drexler, J F" sort="Drexler, J F" uniqKey="Drexler J" first="J F" last="Drexler">J F Drexler</name><name sortKey="Drosten, C" sort="Drosten, C" uniqKey="Drosten C" first="C" last="Drosten">C. Drosten</name><name sortKey="Eickmann, M" sort="Eickmann, M" uniqKey="Eickmann M" first="M" last="Eickmann">M. Eickmann</name><name sortKey="Eis Hubinger, Am" sort="Eis Hubinger, Am" uniqKey="Eis Hubinger A" first="Am" last="Eis-Hübinger">Am Eis-Hübinger</name><name sortKey="Gunther, S" sort="Gunther, S" uniqKey="Gunther S" first="S" last="Günther">S. Günther</name><name sortKey="Helmer, A" sort="Helmer, A" uniqKey="Helmer A" first="A" last="Helmer">A. Helmer</name><name sortKey="Hollmann, B" sort="Hollmann, B" uniqKey="Hollmann B" first="B" last="Hollmann">B. Hollmann</name><name sortKey="Huzly, D" sort="Huzly, D" uniqKey="Huzly D" first="D" last="Huzly">D. Huzly</name><name sortKey="Landt, O" sort="Landt, O" uniqKey="Landt O" first="O" last="Landt">O. Landt</name><name sortKey="Matz, B" sort="Matz, B" uniqKey="Matz B" first="B" last="Matz">B. Matz</name><name sortKey="Monazahian, M" sort="Monazahian, M" uniqKey="Monazahian M" first="M" last="Monazahian">M. Monazahian</name><name sortKey="Niller, H H" sort="Niller, H H" uniqKey="Niller H" first="H H" last="Niller">H H Niller</name><name sortKey="Olschl Ger, S" sort="Olschl Ger, S" uniqKey="Olschl Ger S" first="S" last="Olschl Ger">S. Olschl Ger</name><name sortKey="Reischl, U" sort="Reischl, U" uniqKey="Reischl U" first="U" last="Reischl">U. Reischl</name><name sortKey="Wenzel, J J" sort="Wenzel, J J" uniqKey="Wenzel J" first="J J" last="Wenzel">J J Wenzel</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Panning, M" sort="Panning, M" uniqKey="Panning M" first="M" last="Panning">M. 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